Red-topped tubes have multiple serology applications including: Clotting usually occurs within around 30 minutes. To activate red-topped tubes, phlebotomists must invert them several times to mix the solution. Plastic red-topped tubes typically have clot activators on their interior surfaces, while glass versions don’t. Red stoppers denote draws for serology and immunohematology. Furthermore, ammonium heparin tubes should not be used for ammonia determinations, lithium heparin for lithium measurements and so on. They are inappropriate for any blood banking procedures. However, there are certain situations in which they should not be used. They are suitable for either plasma or whole blood. These anticoagulants activate anti-clotting agents in the blood, blocking coagulation cascades. Green-topped phlebotomy tubes have either ammonium heparin, lithium heparin, or sodium herpin coatings on their inside surfaces. Phlebotomists will select lavender/purple tubes for the following test: Manufacturers design them for direct sampling. ![]() In many cases, there is no need to open purple tubes. They keep white blood cells, platelets and other blood constituents stable (without coagulation) for up to 24 hours. The tubes come pre-lined with various types of EDTA depending on the application. Lavender/purple-topped tubes are for draws that require the use of ethylenediaminetetraacetic acid (EDTA) to bind calcium ions and block coagulation.
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